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. 2021 Dec 17;21(2):100185. doi: 10.1016/j.mcpro.2021.100185

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 6 — Metabolomic analyses of the D492 EMT cell model.A, the metabolome in D492M and D492HER2 was similar compared with that in D492. B, UDP-GlcNAc was expressed higher in D492HER2 compared to the other cell types. C, carbon incorporation into UDP-GlcNAc after 6 h’ culture. Carbons from 1,2-¹³C glucose (Glc) were highly incorporated into UDP-GlcNAc, compared to 5-¹³C glutamine (Gln) and 1-¹³C Gln in all three cell lines. No carbon incorporation from 1-¹³C Gln in all three cell lines. Higher rates of carbon incorporation into UDP-GlcNAc from both 1,2-¹³C Glc and 5-¹³C Gln were observed in D492HER2, compared to D492 and D492M. A higher rate of carbon incorporation into UDP-GlcNAc from 1,2-¹³C Glc was observed in D492M compared with D492. D, knockdown of GFPT2 decreased the production of UDP-GlcNAc in both D492M and D492HER2. E, a decreasing trend for glutamate with GFPT2 knockdown was observed in the D492 EMT model. F, knockdown of GFPT2 significantly decreased cystathionine in all three cell types. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.