Fig. 7.

GFPT2 is a marker for cellular stress.A, gene–metabolite correlation analysis of the NCI60 cancer cell line panel indicated a negative correlation between GFPT2 and GSH. B, GFPT2 RNA expression was significantly upregulated with 2 μM H₂O₂ treatment in MDA-MB-231. C, the total glutathione level did not change after H₂O₂ treatment. D, the GSH level was significantly decreased by the H₂O₂ treatment. E, treatment with 50 mg/l of GSH significantly downregulated the GFPT2 gene expression in MDA-MB-231. F, GSH level was significantly higher in D492 than in D492M and D492HER2. G–N, SQOR RNA expression was significantly downregulated in D492 (G), D492M (H), D492HER2 (I), and MDA-MB-231 (J) by siRNA-mediated knockdown of GFPT2, which was confirmed by the second siRNA (K–N). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.