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. 2021 Oct 13;198(1):11–20. doi: 10.1007/s00442-021-05043-1

Fig. 1.

Fig. 1

Timeline depicting the experimental design of sequential attack of four herbivores and their effect on performance of a fifth herbivore as measure of plant resistance to multi-herbivore attack. On day 0, each Brassica nigra plant was induced with the first set of herbivores (H1) which consisted of either three chewers or six aphids. After five days, all the herbivores were removed from the plant using a brush, and the second set was introduced (H2). This was done for four events of an herbivore sequence. After the herbivores of the last round of herbivory (H4) had been removed from the plant, 10 larvae of Plutella xylostella were introduced on each plant and they were allowed to feed from the induced plants for five days. Each P. xylostella larva was recaptured and weighed as a measure as of plant resistance