Fig. 2.
The AkaLuc and FLuc reporters saturate at different substrate concentrations in vitro. AkaLuc and FLuc expressing MSCs were seeded at a density of 1.5 × 103 cells/well and treated with increasing concentrations of Akalumine-HCl or D-Luciferin (2.5 μM to 5.12 mM). a Representative images of a well plate immediately after the substrate addition to the cells. b, c Light output (flux) as a function of substrate concentration, where b shows the signal obtained from 2.5 μM to 5.12 mM and c from 2.5 μM to 640 μM. Data are displayed as mean ± SD from n = 3. Acquisition parameters: no emission filters, 13.3 cm field of view (FOV), f-stop of 1, binning of 8 and 10 s of exposure