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. 2022 Jan 31;12:1659. doi: 10.1038/s41598-022-05660-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Activin A is highly expressed in tumor-derived cell lines from PDAC patients. (A) Immunofluorescence images of activin A (βA, top panels, AF488) and cytokeratin 19 (CK19, bottom panels, AF488) in hTERT-HPNE, PANC-1, BxPC-3 and MIA PaCa-2 cells. Nuclei were stained with DAPI. Scale bar = 75 µm. (B) Immunoblotting analysis of activin A expression in hTERT-HPNE, PANC-1, BxPC3, MIA PaCa-2 and HeLa cell lysates. (C) Relative INHBA mRNA expression levels in PANC-1 and BxPC-3 cells.