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. 2022 Jan 31;13:584. doi: 10.1038/s41467-022-28082-5

Fig. 6. BLM alters the flexibility of the TRR-ssDNA gate.

Fig. 6

a Representative fluorescence images (from N ≥ 10) of TRR-ssDNA (i) and BTRR-ssDNA (ii), visualized using mCherry/SNAP649 fluorescence (green/red), respectively. A schematic representation of BTRR complexes bound to ssDNA is shown underneath. The scale bar represents 5 µm, and applies to all snapshots. b Representative FD-curves (from N ≥ 10) of TRR-ssDNA (green) and BTRR-ssDNA (pink). For reference, the FD-curve of bare ssDNA is shown in orange. c ‘Subtraction plots’ showing the relative (rel.) lengthening for TRR-ssDNA (green) and BTRR-ssDNA (pink) compared to bare ssDNA. Plots were generated from the FD-curves shown in panel (b). d Representative fluorescence images (from N ≥ 5) of BTRR-ssDNA bound by dsT-DNA. TRR and BLM were visualized using mCherry (i) and SNAP649 (ii) fluorescence, while dsT-DNA was stained with intercalator dye (iii). A schematic representation is shown below. e Representative FD-curves (from N ≥ 5) of BTRR-ssDNA in the absence (pink) and presence (light blue) of dsT-DNA. For reference, the FD-curves of bare ssDNA (orange) and TRR-ssDNA bound by dsT-DNA (dark blue) are also shown. f ‘Subtraction plots’ showing the relative lengthening for dsT-coated TRR-ssDNA (dark blue), BTRR-ssDNA (pink) and dsT-coated BTRR-ssDNA (light blue) compared to bare ssDNA. Plots were generated from the data shown in panel (e). Source data are provided as a Source Data file.