Skip to main content
. 2022 Jan 10;27:916–926. doi: 10.1016/j.omtn.2022.01.006

Figure 3.

Figure 3

UMGD of pHP-hF8/N6 plasmid in HA mice with and without immunomodulation

One hundred μg pHP-hF8/N6 plasmid mixed with 5 vol % NuvOx MB was injected for 30 s with simultaneous pulse-train US exposure (1 s on, 2 s off, 1.1 MHz frequency, 20 cycle pulses, 0.018 ms PD, 2.0 MPa PNP, 13.9 Hz PRF) for 60 s. (A) Structure of the pHP-hF8/N6 plasmid. (B) Long-term FVIII activities in mouse plasma assessed by a modified activated partial thromboplastin time (APTT) assay. (C) Inhibitory antibodies against FVIII were assessed by Bethesda assay. Separate groups of mice were treated the same way except all mice were pretreated with IL-2/IL-2 mAb complexes to modulate immune responses. The treatment schedule is listed below the figure. (D and E) FVIII activities (D) and Inhibitory antibodies (E) against FVIII were examined over time. Each symbol represents data obtained from an individual mouse. Experiments were repeated at least twice.