Fig. 5. Batf3 deficiency favors impaired glucose tolerance, dyslipidemia and liver steatosis in aged mice fed a standard diet.

Glucose levels in serum samples from 30-week-old WT and Batf3^KO mice following fasting (a) or during a glucose tolerance test (b, GTT, n = 18). Percentages of baseline glucose levels in mice of the indicated genotypes during an insulin tolerance test (c, ITT, n = 15). Serum concentrations of basal insulin (d), total cholesterol (e), triglycerides (f), ALT/GPT (g), AST/GOT (h), ALP (I), and GGT (j) in WT and Batf3^KO 30-week-old mice. Quantification of the liver mRNA expression of k gluconeogenesis- (G6Pc and Pck1), l lipolysis- (Cpt1a), and m lipogenesis- (Srbf1, Scd1) related genes in mice of the indicated genotypes. Forty- to 50-week-old mice were used for the quantification of triglycerides in the liver (n) and quantification of the oil red-positive stained area versus the total liver area in OCR (o) liver sections from mice of the indicated genotypes. Bar = 100 μm. Significance was assessed by an unpaired two-tailed Student’s t-test, and Welch’s correction was performed in (n) and (o). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Each point represents a biological replicate. b, c Data are presented as the mean ± SEM, and the same mice were measured repeatedly