Fig. 8.

Women express higher levels of Hsp70 in the amygdala than age-matched men. Olfactory bulb (OB) and amygdala tissues were obtained postmortem from men and women with Lewy body disorders and control subjects via the NIH NeuroBioBank repository (UCLA and University of Miami cohorts) and whole-tissue extracts were subjected to immunoblotting. Age at death (a) and postmortem interval (b) across biological sexes and Lewy body disease status. Values from control and diseased subjects were combined to test the impact of biological sex on Hsp70 levels in the OB (c) or amygdala (g). Values from male and female subjects were combined to test the impact of disease on Hsp70 levels in the OB (d) or amygdala (h). The two-way ANOVA graph showing the separate impacts of sex and disease on Hsp70 levels is in e for the OB and in i for the amygdala. Full-length immunoblots are shown in f and j; the Total Protein Stain was used as the loading control. * p ≤ 0.05; Data in a–b (non-normally distributed by Shapiro–Wilk test) were analyzed by the Kruskal–Wallis test. Data in c–d were analyzed by the two-tailed Mann–Whitney U test. Data in e and i were analyzed by the two-way ANOVA followed by the Bonferroni post hoc. Data in g–h were analyzed by the two-tailed unpaired Student’s t-test. Statistically significant main effects are shown above the graph in i