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. 2022 Jan 31;12:5. doi: 10.1186/s13550-022-00877-z

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Schematic representation of direct T-cell labelling imaging approach. After blood sampling, white blood cells (WBCs) are isolated from peripheral blood mononuclear cells (PBMCs) via density-gradient centrifugation, and incubated with [^99mTc]Tc-HMPAO (shown in the panel) or alternatively with [¹¹¹In]In-oxine. The radiolabelled WBCs are then re-infused in patients and their homing in inflamed organs visualized via single photon emission tomography (SPECT) imaging