FIG. 1.

Sensitivity determination of TTB enrichment-capillary PCR-capillary gel electrophoresis with pure Salmonella enterica serovars Enteritidis, Typhimurium, and Gallinarum. x axis, size in base pairs; y axis, relative fluorescence intensity. Electropherograms: (A) peak patterns of GeneScan 500 size-standard fragments run under denaturing conditions; (B, C, and D) 281-bp PCR product detected from 3, 7, and 9 CFU of pure serovar Enteritidis, serovar Typhimurium, and serovar Gallinarum cultures/ml, respectively. The GeneScan 500 molecular weight marker dye was scaled up five times.