FIG. 3.
Sensitivity determination of TTB enrichment-capillary PCR-capillary gel electrophoresis with chicken intestinal homogenate artificially contaminated with Salmonella serovar Typhimurium. x axis, size in base pairs; y axis, relative fluorescence intensity. Electropherograms: (A) peak patterns of GeneScan 500 size-standard fragments run under denaturing conditions; (B, C, and D) 281-bp PCR product detected from 3 × 103, 3 × 102, and 3 CFU of serovar Typhimurium cultures with chicken intestinal homogenate/ml, respectively. The GeneScan 500 molecular weight marker dye was scaled up four times.