FIG. 4.
Sensitivity determination of TTB enrichment-capillary PCR-capillary gel electrophoresis with chicken intestinal homogenate artificially contaminated with Salmonella serovar Gallinarum. x axis, size in base pairs; y axis, relative fluorescence intensity. Electropherograms: (A) peak patterns of GeneScan 500 size-standard fragments run under denaturing conditions; (B and C) 281-bp PCR product detected from 7 × 102 and 7 CFU of serovar Gallinarum cultures with chicken intestinal homogenate/ml, respectively. The GeneScan 500 molecular weight marker dye was scaled up two times.