Table 1.
Primer sequences, access numbers, and assay IDs for qRT-PCR
| mRNA genes | |||
|---|---|---|---|
| ID | Access# | Primer sequence | Size (bp) |
| NLRP3 | NM_001191642.1 |
F: 5′-TGCTCTTCACTGCTATCAAGC CCT-3′ R: 5′-ACAAGCCTTTGCTCCAGACCC TAT-3′ |
283 |
| ETAR (EDNRA) | NM_012550 |
F: 5′-GTCGAGAGGTGGCAAAGACC-3′ R: 5′-ACAGGGCGAAGATGACAACC-3′ |
64 |
| ETBR (EDNRB) | NM_017333 |
F: 5′-GATACGACAACTTCCGCTCCA-3′ R: 5′-GTCCACGATGAGGACAATGAG-3′ |
86 |
| β-actin | NM_031144.3 |
F: 5′-CGTTGACATCCGTAAAGACCTC-3′ R: 5′-TAGGAGCCAGGGCAGTAATCT-3′ |
302 |
| miRNA genes | |||
| ID | miRBase access# | Target RNA sequence | Assay ID |
| rno-miR-125a-5p | MIMAT0000829 | UCCCUGAGACCCUUUAACCUGUGA | 002,198 |
| rno-miR-501-3p | MIMAT0017198 | AAUGCACCCGGGCAAGGAUUUGG | 464079_mat |
| U6 | Access# NCBI: NR_004394 | ||