REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD49d (α4-integrin/VLA4)	BD Biosciences	Cat# 564397; RRID:AB_2738789
CD11a (LFA-1)	BD Biosciences	Cat# 746310; RRID:AB_2743634
CD62L (L-Selectin)	BD Biosciences	Cat# 746726; RRID:AB_2743990
CD44 antibody	BD Biosciences	Cat# 746710, RRID:AB_2743977
CD4 MicroBeads, human	Miltenyi Biotec	Cat#130-045-101,RRID:AB_2889919
TCR vβ10 PE-conjugated	BD Biosciences	Cat# 553285, RRID:AB_394757
APC-conjugated IgG	BD Biosciences	Cat# 554014, RRID:AB_395209
β-Catenin Antibody	Cell Signaling Technology	Cat# 9562, RRID:AB_331149
Phospho- β-Catenin (Ser675)	Cell Signaling Technology	Cat# 4176, RRID:AB_1903923
β-Actin	Cell Signaling Technology	Cat# 12620, RRID:AB_2797972
Anti-Rabbit secondary antibody	ThermoFisher Scientific	Cat# 31466, RRID:AB_10960844
Biological samples
Cerebrospinal fluid (CSF) from MS patients	Università Cattolica del Sacro Cuore	N/A
Whole blood samples from MS patients and healthy donors	Università Cattolica del Sacro Cuore	N/A
Chemicals, peptides, and recombinant proteins
Pam2CSK4	InvivoGen	tlrl-pm2s-1
Pam3CSK4	InvivoGen	tlrl-pms
LPS-B5 (Ultrapure)	InvivoGen	tlrl-pb5lps
ODN 1585 (CpG)	InvivoGen	tlrl-1585
Critical commercial assays
Cell Trace™ CFSE Cell Proliferation Kit	Invitrogen	C34554
CD4+ CD62L+ T Cell Isolation Kit mouse	Miltenyi Biotec	130-106-643
T Cell Activation/Expansion Kit mouse	Miltenyi Biotec	130-093-627
T Cell Activation/Expansion Kit human	Miltenyi Biotec	130-091-441
Bordetella Pertussis toxin	Sigma-Aldrich	P7208
Freund′s Adjuvant, Complete	Sigma-Aldrich	F5881
PLP (139-151)	Bio-techne	2567/1
Matrigel® Basement Membrane Matrix	Corning	356234
Collagen, Type I	Sigma-Aldrich	C3867
Osteopontin	Sigma-Aldrich	O2260
Cell Counting Kit (CCK)	Sigma-Aldrich	03285
WT Expression Kit	Thermo Fisher Scientific	4411974
GeneChip® WT Terminal Labeling kit	Thermo Fisher Scientific	901524
SYBR® Green Supermix	Biorad	170-8882:
Experimental models: Cell lines
Primary Human CD4+ T cells cultures	This study	N/A
Primary Murine splenocyte cultures	This study	N/A
Experimental models: Organisms/strains
SJL/J	Charles River Laboratories	RRID:IMSR JAX:000686
C57BL/6NCrl	Charles River Laboratories	RRID:IMSR_CRL:027
C57BL/6TLR2−/−	Jackson Laboratories	RRID:IMSR_JAX:004650
C57BL/6MYD88−/−	Jackson Laboratories	RRID:IMSR_JAX:009088
SJL/Jvb10+	Polygene Transgenetics	N/A
Oligonucleotides
All human primers used are listened in Table S1	This paper	N/A
All mouse primers used are listened in Table S2	This paper	N/A
Software and algorithms
Prism 9	GraphPad	https://www.graphpad.com RRID:SCR_002798
Kaluza Software	Beckman Coulter	https://www.beckman.it/flow-cytometry/software/kaluza RRID:SCR_016182
TAC Software	Thermo Fisher Scientific	https://www.thermofisher.com/it/en/home/global/forms/life-science/download-tac-software.html RRID:SCR_016519
I-TASSER	University of Michigan	www.zhanggroup.org/I-TASSER/RRID:SCR_014627
Desmond	D. E. Shaw Research	https://www.deshawresearch.com/resources_desmond.html RRID:SCR_014575
Alliance Q9 Advanced	UVITEC Cambridge	https://www.uvitec.co.uk/alliance-q9-advanced/N/A
SFmap	Paz et al. (2010)	http://sfmap.technion.ac.il/N/A