Figure 2.

Generation of Tm6sf2^-/-rats (line 2) using CRISPR/Cas9 technology. (A) Diagram of CRISPR/Cas9 targeted disruption of rat Tm6sf2 by introducing a deletion of 73 nucleotide into exon 1 (c.11_83del). Exonic and intronic nucleotides are shown in black and green, respectively. The guide RNA (gRNA) binding site and protospacer adjacent motif (PAM) sequence are indicated (top). Genotyping was performed as described in the legend to Figure 1. The amplified PCR fragment was size-fractionated on an agarose gel (bottom). (B) Immunoblot analysis of lysates from livers of 8-week-old female WT and KO rats. CANX, calnexin.