Figure 5.

Lipid accumulation in tissues of Tm6sf2 KOand WT rats. (A) Liver and jejunal tissue sections from chow-fed male WT and KO rats (n = 4/group, 5–6 wk) after a 4-hour fast were stained with Oil Red O and scanned using a NanoZoomer 2.0-HT, digital slide scanner (DM2000), at 10× and 80× magnification. (B) Chow-fed male rats (n = 4/group, 3–4 wk) were fasted for 16 hours and then gavaged with corn oil (10 μL/g body weight). Blood was collected at the indicated times and plasma TG levels were measured (left). TG absorption was calculated by the area under the curve (right). (C) Fecal lipids were extracted from feces of chow-fed female rats (n = 4/group, 13–14 wk) that were collected for 2 days. All experiments were repeated at least once and the results were similar. (D) Male WT and Tm6sf2^-/- rats (n = 4/group, 6 wk) were fasted for 4 hours and then given Triton WR-1339 (500 mg/kg) and 200 μCi of [35S] methionine (1175 Ci/mmol) intravenously and blood was collected at the indicated times and plasma TG was measured. Rats were killed after 120 minutes. To detect [35S] labeled-ApoB, 25 μL plasma was processed as described in the Methods section. Membranes were exposed to Phosphor Screen in Fujifilm BAS cassette 2040 (Fujifilm) for 1 week and signals were quantified using a Storm Imager (PharosFX; Bio-Rad). Circles and bars indicate means ± SD. Differences between groups were analyzed using the Student unpaired 2-tailed t test. ∗∗P < .01, and ∗∗∗∗P < .0001.