Figure 6.

Relative levels of selected mRNAs encoding genes involved in lipid synthesis. Age-matched, chow-fed male WT and Tm6sf2 KO rats (n = 4–5/group, 6–7 wk) were fasted for 4 hours before livers were harvested, and total RNA was isolated as described in the Methods section. Quantitative real-time PCR assays were performed to assay the relative levels of selected mRNAs in livers of the rats. Expression levels were normalized to levels of cyclophilin and compared relative with levels of WT transcript. The official gene symbols were used for all genes. Bars represent means ± SD. The experiment was repeated once and the results were similar.