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. 2021 Dec 16;13(3):879–899. doi: 10.1016/j.jcmgh.2021.12.008

Figure 9.

Figure 9

TM6SF2 is located predominantly in the smooth ER. (A) Liver homogenates (0.25 g) of ad libitum chow-fed male WT and Tm6sf2-/- rats or (B) homogenates from cultured rat hepatocytes (CRL1601 cells) were subjected to Nycodenz continuous density gradient centrifugation as described in the Methods section. Equal proportions (∼1%) of each fraction were subjected to immunoblotting for the indicated organelle markers. (C) Schematic of protocol used to separate rough and smooth ER from primary rat hepatocytes as described in the Methods section (top). Immunoblot analysis was performed using equal proportions (∼1%) of each fraction (bottom). ∗Nonspecific band. All experiments were repeated at least twice, and results were similar. CANX, calnexin; cGN, cis-Golgi; cyt, cytosol; GOS28, Golgi SNAP receptor complex member 1; H, homogenate; LDH, lactate dehydrogenase; PNS, postnuclear supernatant; rER, rough ER; RPN1, ribophorin 1; sER, smooth ER; tGN, trans-Golgi; VTI1A, vesicle transport through interaction with t-SNAREs homolog 1A.