Table 2.
TM6SF2-Interacting Proteins Identified by MS
| Protein | Spectral counts |
Spectral index |
||
|---|---|---|---|---|
| Luc | hTM6 | Luc | hTM6 | |
| Apolipoprotein B | – | 281.48 | – | 2.33E-05 |
| Myosin-11 | 52.79 | 2.64E-06 | ||
| ATP-binding cassette subfamily D member 3 | – | 51.00 | – | 1.29E-05 |
| Myosin-1 | 48.39 | 2.39E-06 | ||
| Mitochondrial inner-membrane protein | – | 37.51 | – | 5.55E-06 |
| Myosin-8 | 34.42 | 1.84E-06 | ||
| Long chain fatty acid CoA ligase 5 | – | 28.00 | – | 6.00E-06 |
| Carnitine O-palmitoyltransferase 1 | – | 26.00 | – | 3.21E-06 |
| Bile acyl-CoA synthetase | – | 23.87 | – | 4.80E-06 |
| ATP synthase subunit β | – | 20.00 | – | 3.44E-06 |
| 3-ketoacyl-CoA thiolase | – | 20.00 | – | 4.42E-06 |
| Very-long-chain enoyl-CoA reductase | – | 20.00 | – | 8.62E-06 |
| Myosin light chain 3 | – | 17.02 | – | 2.02E-05 |
| Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 1 | – | 16.00 | – | 3.91E-06 |
| MOSC domain-containing protein 1 | – | 15.50 | – | 8.82E-06 |
| Cytochrome b-c1 complex subunit 2 | – | 15.00 | – | 1.63E-06 |
| Heat shock protein heat shock protein 90-β | – | 13.94 | – | 1.78E-06 |
| Mitochondrial glutamate carrier 1 | – | 13.89 | – | 4.21E-06 |
| Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 | - | 13.00 | - | 1.03E-06 |
| Actin, α | - | 13.00 | - | 3.34E-06 |
NOTE. Proteins with spectral count greater than 13 are listed above. Spectral counts are the number of peptide spectral matches in a sample, where a peptide spectral match is the number of MS/MS spectra that the software was able to match to a peptide from that protein. Spectral index is a value used for comparing protein amounts between samples, and is calculated based on fragment ions that are matched to peptides from the protein, which then are normalized based on protein amount and protein molecular weight. –, undetectable.
ATP, adenosine triphosphate; hTM6, human V5-tagged TM6SF2; Luc, luciferase; MOSC, mitochondrial amidoxine reducing component 1.