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PCR of M. gallisepticum reference with outer primers pvpA 5′ and pvpA 3′. Molecular weight markers (Life Technologies, Rockville, Md.) are in the unmarked lane on the left (sizes on the left are in base pairs). Lane 1, 6/85; lane 2, field isolate (CK/CA/96/1); lane 3, R; lane 4, ts-11; lane 5, F; lane 6, K503; lane 7, K703; lane 8, K730; lane 9, A5969; lane 10, negative control. Size variation of amplification products from 497 bp (lanes 3, 4, and 9) to 267 bp (lane 5) was observed.
