TABLE 3.
RFLP and sequencing analysis of the C-terminus-encoding region of the pvpA gene from field isolates
| Field strain | PCR product sizea | RFLP groupb | % Sequence identityc
|
|
|---|---|---|---|---|
| Nucleotide | Amino acid | |||
| TK/GA/73/1 | 497 | A | 99.8 | 99.4 |
| CK/CO/84/1 | 497 | A | 100 | 100 |
| CK/GA/98/1 | 488 | B | 98.5 | 96.9 |
| CK/NC/99/1 | 497 | B | 100 | 100 |
| CK/NC/99/2 | 497 | B | 100 | 100 |
| CK/NC/99/3 | 497 | B | 100 | 100 |
| TK/MI/97/1 | 437 | D | 100 | 100 |
| TK/MI/97/2 | 437 | D | 100 | 100 |
| TK/NB/95/1 | 437 | D | 100 | 100 |
| TK/NB/95/2 | 437 | D | 100 | 100 |
| TK/OH/97/1 | 437 | D | 100 | 100 |
| TK/NC/95/1 | 437 | D | 100 | 100 |
| CK/CA/96/1 | 437 | E | 100 | 100 |
| TK/VA/96/1 | 437 | F | 98.8 | 97.9 |
| TK/CO/98/1 | 437 | F | 98.1 | 96.5 |
| TK/CO/98/2 | 437 | F | 98.1 | 96.5 |
| TK/CO/98/3 | 437 | F | 98.1 | 98.8 |
| HF/MA/94/1 | 437 | F | 100 | 100 |
| HF/PA/95/1 | 437 | F | 100 | 100 |
| HF/TN/96/1 | 437 | F | 100 | 100 |
| GF/SC/97/1 | 437 | F | 100 | 100 |
| HF/MA/98/1 | 437 | F | 100 | 100 |
| HF/TX/97/1 | 266 | H | 96.1 | 91.9 |
| CK/AK/99/1 | 437 | I | 98.4 | 96.5 |
a
Size in base pairs, estimated from molecular weight marker and sequence analysis.
b
RFLP groups correspond to M. gallisepticum reference strains as follows: A, R; B, ts-11; D, 6185; E, S6; F, HF-51; H, F.
c
Sequence identity with corresponding RFLP group M. gallisepticum reference strain.