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Comparative analysis of clinical samples from commercial broiler-breeder flocks
| Flock age (wk) | No. of positive samples/no. tested by:
|
Isolation | |
|---|---|---|---|
| M. gallisepticum PCRa | pvpA PCRb | ||
| 50 | 8/10 | 4/10 | M. gallisepticum, M. synoviae, M. gallinarum |
| 58 | 10/10 | 1/10 | NTc |
| 58 | 4/4 | 4/4 | NT |
| 40 | 5/5 | 2/5 | M. gallisepticum, M. synoviae, M. gallinarum |
PCR using primers MG13 and MG14, directed to the rRNA gene of M. gallisepticum.
Seminested pvpA PCR.
NT, not tested.
