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. 2022 Jan 18;12:773150. doi: 10.3389/fphar.2021.773150

FIGURE 4.

FIGURE 4

Micheliolide (MCL) inhibited irradiation-induced NLRP3 inflammasome activation in mice (A) Representative small intestine hematoxylin and eosin staining from NLRP3−/− and wild-type (WT) mice following irradiation (B) Serum IL-1β levels in NLRP3−/− and WT mice following irradiation (C) Serum IL-18 levels in NLRP3−/− and WT mice following irradiation (D) Serum TGF-β1 levels in NLRP3−/− and WT mice following irradiation (E) Serum TNF-α levels in NLRP3−/− and WT mice following irradiation (F) Serum IFN-γ levels in NLRP3−/− and WT mice following irradiation (G) Caspase-1 activity in the small intestine of NLRP3−/− and WT mice treated with MCL following irradiation (H) Expression of pro-casp1, casp1 p20, GSDMD, and GSDMD-N in NLRP3−/− and WT mice treated with MCL following irradiation (I) Formation of the NLRP3 and ASC complex in NLRP3−/− and WT mice treated with MCL following irradiation (J) Representative image of immunofluorescencent co-localization (arrows) between NLRP3 and ASC in small intestine tissue. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, two-tailed Student’s t-test. Con, untreated WT mice; IR, WT mice that were treated with irradiation (10 Gy); MCL + IR, mice pre-treated with MCL prior to irradiation (10 Gy); NLRP3−/− + IR, NLRP3 knockout mice exposed to radiation (10 Gy); IL, interleukin; NLRP3, nucleotide binding domain leucine-rich repeat-containing receptor-pyrin domain containing three; GSDMD, gasdermin D. N = 15/group.