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. 2022 Jan 18;12:773150. doi: 10.3389/fphar.2021.773150

FIGURE 5.

FIGURE 5

MCL enhances autophagy in mice intestinal tissue and HIEC after radiotherapy. Con, untreated HIECs; IR, HIECs that were treated with irradiation (10 Gy); MCL + IR, HIECs pre-treated with MCL (10 μM) prior to irradiation (A) Representative immunofluorescence image of LC3 aggregation in HIECs (B) Autophagosomes (arrows) in HIEC detected by transmission electron microscopy (C) Western blot of p62, beclin 1, and LC3 protein expression in HIEC. Con, untreated WT mice; IR, WT mice that were treated with irradiation (10 Gy); MCL + IR, WT mice pre-treated with MCL (50 mg/kg) prior to irradiation (10 Gy) (D) Immunohistochemical staining of p62, beclin 1, and LC3B in small intestine tissues (E) Autophagosomes (arrows) in small intestine tissue detected by transmission electron microscopy (F) Western blot of p62, beclin 1, and LC3 protein expression in small intestine tissues. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, two-tailed Student’s t-test. IR, irradiation; HIEC, human intestinal epithelial cell; MCL, micheliolide.