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. 2022 Jan 18;12:747325. doi: 10.3389/fphar.2021.747325

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Copyright © 2022 Gao, Chen, Fu, Yin, Wang, Sun, Ren and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of KD on ethanol-induced liver inflammation and hepatocyte apoptosis in mice. (A) Serum TNF-α levels. (B) Serum IL-6 levels. (n = 5–7). (C) Immunohistochemistry staining for F4/80 and CD3 in the mice liver. (D) Expression of apoptosis-related proteins determined by western blotting. (E) Quantitative analysis of protein expression (n = 3). GAPDH was used as a loading control. (F) TUNEL-stained liver sections. Scale bar: 50 µm. Data represent the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. the model group.