Skip to main content
. 2022 Jan 18;12:772926. doi: 10.3389/fphar.2021.772926

FIGURE 3.

FIGURE 3

PBK suppressed the migration and invasion of CRC cells with stabilizing CDH1. A and B, results of migration and invasion assays showing exogeneous expression of PBK suppressed the migration and invasion of CRC cells. Bar = 100 µm. Assays were performed in triplicate. Data are shown as mean ± S.D. *, p < 0.05, **, p < 0.01. (C), immunoblot and RT-PCR analysis showing up-regulated CDH1 without altering CDH1 in PBK induced CRC cells. (D), PBK suppressed the TOP/FOP reporter activity in CRC cells. Assays were performed in triplicate. Data are shown as mean ± S.D. *, p < 0.01. (E), protein expression of CDH1 and apoptosis-related proteins in CRC cells after OTS514 treatment. Note that BCL2 was expressed at under detectable levels in HCT116 and Caco-2. Bax expression was under detectable level in LoVo. Numbers below the immunoblot bands indicate relative expression.