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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Cell Rep. 2021 Apr 6;35(1):108933. doi: 10.1016/j.celrep.2021.108933

Figure 5. PNGS sequon engineering is applicable to Env trimers produced in cells from different species and derived from diverse HIV-1 strains.

Figure 5.

(A) Change in PNGS occupancy between WT proteins produced in ExpiCHO-S cells upon introduction of NxT T135 T158S. The presented data represents the arithmetic difference between the glycan occupancy of the NxT protein minus the WT glycan occupancy, representing a percentage point change (p.p.). A positive p.p. change represents a higher occupancy of the NxT variant compared to the WT. Sites labeled with an asterisk represent those for which data could not be obtained for either the WT or the NxT variant.

(B–D) For additional Env sequences the data is displayed in an identical manner for (B) AMC009, (C) AMC011, and (D) HxB2. N411 was introduced into HxB2 NxT but is not present in WT and is marked as an asterisk.