Table 1.

Occurrence of NxT and NxS sequons in the BG505 sequence compared to 6,516 unique Env sequences found in the Los Alamos database

			Frequency in natural isolates^a			Glycan occupancy (%)
Glycan	BG505	Motif	% NxT	% NxS	% no PNGS

N88	NxT	NVT	98.2	0.4	1.4		>95
N133	NxT	NVT	15.0	3.0	82.0	75
N137	NxT	NIT	20.1	3.5	76.4	>95
N156^*	NxS^*	NCS^*	8.6^*	87.6^*	3.8^*	>95^*
N160	NxT	NMT	88.9	2.1	9.0	>95
N190^*	NxS^*	NRS^*	ND^*,b	ND^*,b	ND^*,b	80^*
N190c^*	NxS^*	NNS^*	ND^*,b	ND^*,b	ND^*,b	75^*
N197^*	NxS^*	NTS^*	1.1^*	96.8^*	2.1^*	90^*
N234	NxT	NGT	74.8	5.0	20.2	>95
N262^*	NxS^*	NGS^*	0.2^*	98.9^*	0.9^*	>95^*
N276	NxT	NIT	76.5	18.5	5.1	>95
N295	NxT	NCT	58.2	1.5	40.4	>95
N301	NxT	NNT	93.7	0.2	6.2	>95
N332^*	NxS^*	NVS^*	3.0^*	68.5^*	28.5^*	>95^*
N339	NxT	NET	65.1	0.1	34.8	>95
N355	NxT	NNT	72.8	1.6	25.5	>95
N363^*	NxS^*	NSS^*	0.4^*	7.7	91.9^*	>95^*
N386^*	NxS^*	NTS^*	40.0^*	46.8^*	13.2^*	>95^*
N392	NxT	NST	70.0	9.2	20.8	>95
N398^*	NxS^*	NTS^*	11.0^*	2.9^*	86.1^*	>95^*
N406	NxT	NST	2.7	0.5	96.8	>95
N411^*	NxS^*	NDS^*	44.4^*	1.3^*	54.3^*	>95^*
N448	NxT	NIT	87.4	0.1	12.5	>95
N463	NxT	NST	ND^b	ND^b	ND^b	>95
N611^*	NxS^*	NSS^*	11.6^*	86.8^*	1.6^*	40^*
N618^*	NxS^*	NLS^*	16.6^*	75.3^*	8.1^*	>95^*
N625	NxT	NMT	96.2	0.0	3.8	>95
N637	NxT	NYT	92.5	3.8	3.7	>95

The occupancy of each PNGS on the BG505 SOSIP.v4.1 trimer is also recorded.

Indicates the PNGS that have an NxS motif.

Data obtained from 6,516 unique HIV envelope sequences found in the Los Alamos database (https://www.hiv.lanl.gov).

PNGS are located in highly variable regions and the specific sites differ between the natural isolates, making reliable calculations not possible.