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. 2021 Jan 14;107(2):446–456. doi: 10.3324/haematol.2020.269142

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Figure 8. — Impaired erythroid differentiation of RPS19-deficient CD34⁺ cord blood cells can be rescued by the EFS-RPS19 vector. (A) RPS19 mRNA expression in CD34⁺ cord blood cells transduced with shRNA. (B) Percentage of GFP^high population in RPS19-deficient CD34⁺ cord blood cells treated or not with EFS-RPS19 during erythroid differentiation from stage I to stage III. (C) Fluorescnce activated cell sorting analysis of erythroid differentiation of RPS19-deficient cells treated or not with EFS-RPS19 on day 16. (D) Percentage of indicated cell outputs of GFP^high populations on day 16. (E) Red blood cell pellets at the end of stage III initiated with equal numbers of CD34⁺ cord blood cells (data shown as mean ± standard deviation, ^P<0.05 compared to the shRNA1 group, ^#P<0.05 compared to the shRNA2 group, *P<0.05, **P<0.01, ***P<0.005 by a t-test, 3 independent experiments).