FIGURE 4.

Rs10889602T/T SNP deletion in A549 cells decreases PDE4B and COL1A1 mRNA and/or protein expression levels. (A) qRT-PCR analysis of PDE4B mRNA expression levels. GAPDH was the loading control. (B) Western blot analysis of PDE4B protein expression levels, β-actin was the loading control. (C) Densitometric quantification of western blot results of PDE4B expression levels. (D) qRT-PCR analysis of COL1A1 mRNA expression levels, GAPDH was the loading control. Data are expressed as mean ± standard error (mean ± SEM), n = 6 for each group, multiple comparisons of different A549 cell lines were done using the one-way ANOVA with Bonferroni post hoc test. p-value < 0.05 was considered statistically significant (**p < 0.01 and ***p < 0.001). qRT-PCR, quantitative real-time polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RQ, relative quantity.