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. 2022 Jan 12;12:775797. doi: 10.3389/fgene.2021.775797

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Copyright © 2022 Zhao, Chen, Zhou, Reinach, Wang, Juo, Yang, Xue, Shi, Liang, Zeng, Qu and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The Effect of PDE4B Knockdown on Expression of Type I collagen (COL1A1) in cultured HSFs. The HSFs were treated with siRNA for 48 h, then the knockdown efficiency of PDE4B and the expression of COL1A1 were analyzed by Western blot (A). (B,C) The bar graphs represent relative protein levels of PDE4B and COL1A1. (B) The protein expression level of PDE4B. (C) The protein expression level of COL1A1. Mock: blank control (transfection reagent only); NC, negative control (cells transfected with scrambled siRNA). β-actin was the loading control. Data were expressed as mean ± standard error (mean ± SEM), n = 5 for each group. Comparison of the PDE4B siRNA-transfected group and scrambled siRNA group were determined by using one-way ANOVA. p-value < 0.05 was considered statistically significant (***p < 0.001).