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Safranin O staining of the explanted tissue samples. (A) Control native nasal cartilage samples stained with safranin O revealed characteristic septal hyaline cartilage rich in glycosaminoglycans (GAGs). (B and C) In comparison, there were no GAGs in 2- and 6-month newly formed tissue (NFT) samples at the place of biopsy and the defect site was visible. Red stain indicates the presence of cartilage-specific proteoglycans. Scale represents 2.5 mm.
