Figure 7.

(A) In vitro adipogenic differentiation of GP-CPs in 2-dimensional cultures. Oil red O staining of intracellular heterogeneous lipid vacuoles in Growth plate – cultures of chondroprogenitors (GP-CPs) without and after adipoinduction. Scale bar equals 100 µm. (B) Assessment of early and late adipogenic genes revealed significantly increased expression of PPARG and highly increased LPL expression compared with basal levels. Under the adipogenic program, ALP levels were not significantly altered, whereas RUNX2 was significantly downregulated (*P < 0.05; n = 4).