Effect of LINC01534 on the metabolic dysfunction of chondrocytes induced
by interleukin-1β (IL-1β). (A) Detection of the expression
of LINC01534 in chondrocytes transfected with si-LINC01534 or siNC by
quantitative real-time polymerase chain reaction (qRT-PCR).
(B) Detection of the mRNA expressions of aggrecan and
collagen II in chondrocytes by qRT-PCR. (C) Detection of
the mRNA expressions of matrix metalloproteinase–3 (MMP-3), MMP-9, and
MMP-13 in chondrocytes by qRT-PCR. (D, E)
Detection of the protein expressions of MMP-3, MMP-9, and MMP-13 in
chondrocytes by Western blot. (F, G) Detection of the contents of MMP-3,
MMP-9, and MMP-13 in chondrocyte supernatant by enzyme-linked
immunosorbent assay (ELISA). *P < 0.05.