Figure 1.

Time-dependent effects of TA and CD, alone or in combination with HA-CTL mixture on human chondrocyte viability. A total of 7000/cm² cells were seeded in 24-well culture dishes and treated with TA, CD, or their combination, in the presence or absence of HA-CTL (A) CD, (B) CD + TA at different combinations, (C) CD + TA in the presence or absence of HA-CTL mixture. The MTT test was performed at 1, 3, and 6 days after treatment. In (D) the viability of treated cells at 6 days was expressed as percentage of untreated controls. Each experiment was performed in triplicate, using different cell preparations taken from different donors. Data are reported as mean ± SD of the 3 independent experiments. Statistical differences based on unpaired Student’s t test. *P < 0.05 versus untreated cells. TA = triamcinolone acetonide; CD = hydroxypropyl-β-cyclodextrin; HA = hyaluronic acid; CTL = chitosan (Chitlac).