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. 2020 Feb 28;13(2 Suppl):920S–924S. doi: 10.1177/1947603520908658

Figure 2.

Figure 2.

Chondrocyte expression of pro-inflammatory molecules after exposure to TA + CD in the presence or absence of HA-CTL mixture. Human chondrocytes were exposed for 24 hours to CM of activated U937 and then cultured with the studied molecules. RNA transcript levels specific for IL-1β and IL-6 were evaluated by qPCR. Activated U937 cell expression of (A) IL-1β and (B) IL-6. Human chondrocytes expression for (C) IL-1β and (D) IL-6. c1: 1.5 mg/mL CD and 21 µg/mL TA; c2: 5 mg/mL CD and 70 µg/mL TA; mix1: c1 + HA-CTL; mix2: c2 + HA-CTL. Each experiment was performed in triplicate, using different cell preparations taken from different donors. Statistical differences are based on one-way ANOVA test and data are expressed as mean ± SD obtained from the 3 independent experiments. CM = chondrocytes cultures exposed to U937 CM. *P < 0.05, **P < 0.001, and ***P < 0.0001 versus respective CM treated control. §§P < 0.001 versus respective chondrocyte culture treatment. TA = triamcinolone acetonide; CD = hydroxypropyl-β-cyclodextrin; HA = hyaluronic acid; CTL = chitosan (Chitlac).