Table 2.
Antibodies.
| Antibody | Clonality | Clone Name | Company Code | Dilution Factor | Company |
|---|---|---|---|---|---|
| Ihh | Rat monoclonal | 3A11 | GTX53235 | 2 μg/mL (WB)■20 μg/mL (IHC) | Genetex |
| PTHrP | Rabbit polyclonal | PA5-40799 | 1 μg/mL (WB) · 8 μg/mL (IHC) | Thermofisher Scientific | |
| Gli1 | Rabbit polyclonal | ab167388 | 1 μg/mL | Abcam | |
| Gli2 | Rabbit polyclonal | ab167389 | 1 μg/mL | Abcam | |
| Gli3 | Rabbit polyclonal | ab69838 | 1 μg/mL | Abcam | |
| Lrp5/6 | Rabbit polyclonal | ab51910 | 2 μg/mL | Abcam | |
| βcatenin | Mouse monoclonal | 12F7 | ab22656 | 2 μg/mL | Abcam |
| βcatenin(phos) | Rabbit polyclonal | ab27798 | 2 μg/mL | Abcam | |
| sfrp2 | Mouse monoclonal | C-4 | sc365524 | 2 μg/mL | Santa Cruz Biotechnology |
| sfrp3 | Goat polyclonal | PA5-47793 | 0.1 μg/mL | Thermofisher Scientific | |
| Nkx3.2 | Rabbit polyclonal | PA5-21108 | 2 μg/mL | Thermofisher Scientific | |
| Col2a1 | Rabbit polyclonal | SC28887 | 20 μg/mL (IHC) | Santa Cruz Biotechnology | |
| Col10 | Mouse monoclonal | X53 | 14-9771-82 | 10 μg/mL (IHC) | Invitrogen |
WB = Western blot; IHC = immunohistochemistry.