Fig. 2.

Generation of transgenic mice with the conditional EGFRvIII-CAR allele at the Rosa26 locus (A) The Rosa26 chromosome 6 locus and the location of the targeted insertion. Restriction sites for EcoRI and BgII enzymes and locations for Probes A and B are indicated. (B) Correct genomic targeting of the transgene at the 3′ end was confirmed by Southern blotting of BgII-digest genomic DNA with Probe B. (C) Overview of targeting the conditional allele to the Rosa26 locus of C57BL/6J embryonic stem (ES) cells and generation of founder mice.