TABLE 2.
Sequences and locations of primers used in this study
| Primer | Sequence | Annealing temp (°C) | Location | Source or reference |
|---|---|---|---|---|
| DJA | TCCATCCCATACCTGCA | 55 | NS1 | |
| DJS | GACATGGGGTATTGGAT | 24 | ||
| DV1 | GGRACKTCAGGWTCTCC | 50 | NS3 | |
| DV3 | AARTGIGCYTCRTCCAT | 8 | ||
| FG1 | TCAAGGAACTCCACACATGAGATGTACT | 45 | NS5 | |
| FG2 | GTGTCCCATCCTGCTGTGTCATCAGCATACA | 15 | ||
| CFDJ 9977 | GCATGTCTTCCGTCGTCATCC | 55 | NS5 | |
| FUDJ 9166 | GATGACACAGCAGGATGGGAC | 7 | ||
| VD8 | GGGTCTCCTCTAACCTCTAG | 53 | 3′ Nontranslated region | |
| EMF1 | TGGATGACSACKGARGAYATG | NS5 | 26 | |
| cFD2 | GTGTCCCAGCCGGCGGTGTCATCAGC | 53 | NS5 | |
| MA | CATGATGGGRAARAGRGARRAG | 19 | ||
| MAMD | AACATGATGGGRAARAGRGARAA | 53 | NS5 | This article |
| FS 778 | AARGGHAGYMCDGCHATHTGGT | 54 | NS5 | This article |