TABLE 3.
Comparison between different RT-PCR assays for detection of the Flavivirus genus
| Primer pair | Titer (log TCID50 ml−1) | Amplification of Flavivirus genus virusa
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DEN-1 | DEN-2 | DEN-4 | JE | USU | WN a | WN b | WSL | YF FNV | YF 17D | Zika | ||
| DJA/DJS | 5 | + | + | + | − | − | + | − | − | − | − | + |
| DV1/DV3 | 5 | + | + | + | − | − | + | − | − | − | − | + |
| CFDJ 9977/FUDJ 9166 | 5 | + | + | − | − | − | − | + | − | − | − | − |
| VD8/EMF1 | 5 | − | − | − | − | − | − | − | − | − | − | − |
| FG1/FG2 | 5 | + | + | + | − | + | − | + | − | − | + | + |
| cFD2/MA | 5 | + | + | + | + | + | + | + | + | + | + | + |
| cFD2/MA | 4 | − | − | − | − | − | − | − | − | − | − | − |
a
+, positive amplification; −, negative amplification. USU, Usutu virus; WN a, strain E101; WN b, local strain; WSL, Wesselsbron virus.