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. 2021 Jul 14;24(2):229–244. doi: 10.1093/neuonc/noab175

Fig. 2.

Fig. 2

The effect of KPT-9274 on glioma cell viability. (A) Cell viability assays depicting the effect of KPT-9274 on the adherent LN229, U251-HF, U373, A172, U87, and T98G cells and the patient-derived glioma stem-like cell (GSC) lines GSC262, GSC811, GSC11, GSC23, GSC20, GSC272, GSC5-22, and BT142. (B) Comparison of half-maximal inhibitory concentration (IC50) values for adherent glioma cells and GSCs. (C) The ability of KPT-9274 to induce apoptosis in GSC811, U251-HF, and GSC5-22 cell lines 24, 48, and 72 h after treatment as assessed by Annexin V propidium iodide flow cytometry and indicated as proportions of live, pre-apoptotic, apoptotic, and necrotic cells. (D) Measurement of caspase 3/7, caspase 8, and caspase 9 activity in the U251-HF, GSC262, GSC811, and GSC5-22 cell lines by Caspase Glo 3/7, Caspase Glo 8, and Caspase Glo 9 assays, respectively. Abbreviations: WT, wild type, OD450, optical density measured at wavelength 450 nM, RLU, relative light unit.