Fig. 6.

The effect of NAMPT inhibition on ex vivo slice cultures of glioma tissue. (A) Schematic representation of method to use for organotypic slice culture experiments (B) NAD levels after KPT-9274 or vehicle treatment of mouse organotypic glioma xenograft tissue slices. (C) NAD levels after KPT-9274 or vehicle treatment of human organotypic glioblastoma tissue slices obtained during surgery. (D) Hematoxylin and eosin (H&E) stain (left panels) and SirT1 (middle panels) and cleaved PARP (right panels) immunohistochemistry specimens showing expression in vehicle- or KPT-9274-treated human organotypic glioma slice culture samples. Scale bar = 200 µm (E) Graphic depiction of the impact of NAMPT inhibition on cellular energy-dependent pathways. Abbreviation: OD₅₉₅, optical density measured at wavelength 595 nM.