TABLE 4.
E. coli strains used in this study
| Strain | Genotype and/or characteristics | Reference |
|---|---|---|
| E. coli | ||
| 536 | pyelonephritis isolate 536; pks+, HPI+ (O6:K15:H31) | 47 |
| 536-HDM | 536ΔhlyI, ΔhlyII::cat | 48 |
| 536-HDM csrA51 | 536-HDM csrA51::cat | This study |
| BL21 (DE3) | F-, gal met r-m-hdsS λlysPlacUV5-T7-Gen1 PlacIqlacI | 91 |
| DH5α | F-endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(argF-lacZYA) U169 (Φ60ΔlacZ M15λ-) | 92 |
| IHE3034 | newborn-meningitis isolate; pks+, HPI+ (O18:K1:H7) | 93 |
| M1/5 | Fecal isolate of a healthy individual; pks+, HPI+, Strr | 42 |
| M1/5 csrA51 | M1/5 csrA153::FRT-kan-FRT; Kanr, Strr | This study |
| M1/5 ΔuvrY | M1/5 ΔuvrY::FRT-cat-FRT; Cmr, Strr | This study |
| M1/5 HPI- | M1/5 Δ(ybtA-fyuA)::FRT; Strr | This study |
| M1/5 HPI- csrA51 | M1/5 Δ(ybtA-fyuA)::FRT csrA153::FRT-kan-FRT; Kanr, Strr | This study |
| M1/5 Kan-PlacUV5-clbQ'-'venus | M1/5 ΔPclbQ::(FRT-kan-FRT-PlacUV5) ΔclbQ4-723::(venus-cat); Cmr, Kanr, Strr | This study |
| M1/5 PclbQ-AL-venus | M1/5 Δ(clbQ 5′UTR-clbQ)::(artificial 5′UTR-venus-cat); Cmr, Strr | This study |
| M1/5 PclbQ-AL-venus csrA51 | M1/5 Δ(clbQ 5′UTR-clbQ)::(artificial 5′UTR -venus-cat) csrA153:: FRT-kan-FRT; Cmr, Kanr, Strr | This study |
| M1/5 PclbQ-clbQ'-'venus | M1/5 ΔclbQ4-723::(venus-cat); pks+, HPI+, Cmr, Strr | This study |
| M1/5 PclbQ-clbQ'-'venus csrA51 | M1/5 ΔclbQ4-723::(venus-cat) csrA153:: FRT-kan-FRT; Cmr, Kanr, Strr | This study |
| M1/5 PlacUV5-clbQ'-'venus | M1/5 ΔPclbQ::(FRT-PlacUV5) ΔclbQ4-723::(venus-cat); Cmr, Strr | This study |
| M1/5 PlacUV5-clbQ'-'venus csrA51 | M1/5 ΔPclbQ::(FRT-PlacUV5) ΔclbQ4-723::(venus-cat) csrA153::FRT-kan-FRT; Cmr, Kanr, Strr | This study |
| M1/5 PlacUV5-clbQ*'-'venus | M1/5 ΔPclbQ::(FRT-PlacUV5) ΔclbQ4-723::(venus-cat); carries modified nucleotides in clbQ 5′UTR; Cmr, Strr | This study |
| M1/5 PlacUV5-clbQ*'-'venus csrA51 | M1/5 ΔPclbQ::(FRT-PlacUV5) ΔclbQ4-723::(venus-cat) csrA153::FRT-kan-FRT; carries modified nucleotides in clbQ 5′UTR; Cmr, Kanr, Strr | This study |
| M1/5 PlacUV5-ybtA'-'venus | M1/5 ΔPybtA::(FRT-PlacUV5) ΔybtA4-960::(venus-cat); Cmr, Strr | This study |
| M1/5 PybtA-ybtA'-'venus | M1/5 ΔybtA4-960::(venus-cat); Cmr, Strr | This study |
| MG1655 | K-12 F– λ– ilvG– rfb-50 rph-1; pks–, HPI– | 94 |
| SP15 | neonatal meningitis isolate, pks+, HPI+, Strr | 95 |
| SP15 ΔuvrY | SP15 ΔuvrY::cat; Cmr, Strr | This study |
| SP15 ΔcsrB | SP15 ΔcsrB::cat; Cmr, Strr | This study |
| SP15 ΔcsrC | SP15 ΔcsrC::tet; Strr, Tetr | This study |
| SP15 ΔcsrB ΔcsrC | SP15 ΔcsrB::FRT ΔcsrC::tet; Strr, Tetr | This study |
| SY327λpir | λ(lac pro) argE (Am) rif nalA recA56 (λpir) | 96 |
| Salmonella enterica Typhimurium | ||
| WR1542 | fepA::Tn10dTc, iroN::pGP704 cir::MudJ carrying plasmid pACYC5.3L; Apr, Cmr, Kanr, Tcr | Gift from W. Rabsch, Wernigerode |