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. 2021 Dec 16:1–12. doi: 10.1159/000520221

Fig. 4.

Fig. 4

Detection of SARS-CoV-2 N-protein by immunohistochemistry and RNA by ISH. Top left: positivity for viral N-protein in hyaline membranes and in the endothelial compartment; top right: positivity for viral RNA in ALVs, endothelia and hyaline membranes. Scale bars denote 100 μm. Bottom: double staining with N-protein (red) and ERG (brown) reveals close association of viral proteins and the END (black arrows: endothelial cell surface coated with N-protein). SARS-CoV-2, severe acute respiratory syndrome coronavirus-2; N-protein, nucleocapsid protein; ISH, in-situ hybridization; END, endothelium; ALV, alveolar pneumocyte; ERG, ETS-related gene.