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Mycological nail sampling. After cleaning the nail with soap and water and alcohol, the nail plate is clipped with a double-action nail nipper. A #1 curette is used to slowly and carefully scrape the subungual debris for analysis by KOH with direct microscopy, fungal culture, and/or PCR. Use of a black background provides contrast to ensure sufficient sample is collected (see also online suppl. Video 1). KOH, potassium hydroxide; PCR, polymerase chain reaction.
