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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Cell Rep. 2022 Jan 11;38(2):110217. doi: 10.1016/j.celrep.2021.110217

Figure 4. HA-8mer follicular accumulation and immunogenicity are dependent on MBL.

Figure 4.

(A and B) C57Bl/6 or MBL KO mice (n = 5/group) were immunized with 5 μg AlexaFluor 647-labeled influenza HA-8mer particles and saponin adjuvant. Draining lymph nodes were harvested on day 7 and cleared for confocal imaging. Shown are total HA-8mer signal per Z plane of cleared tissues (A) and percent HA-8mer signal found within follicles (B). Error bars indicate SEM; points represent average values between paired draining lymph nodes from one animal; **, p <0.01, ns = not significant by Mann-Whitney test.

(C) C57Bl/6 or MBL KO mice (n = 5/group) were immunized with 5 μg influenza HA-8mer particles and saponin adjuvant. Shown are serum hemagglutinin-specific IgG titers 5 and 6 weeks post immunization. Error bars indicate SEM; **, p <0.01 by Mann-Whitney test.

(D and F) Absolute counts of total GC B cells (D, *, p <0.05 by Mann-Whitney test) and absolute counts of antigen-specific GC B cells at day 12 (E, **, p <0.01 by Mann-Whitney test) and average MFI of antigen specificity stain among antigen-specific GC B cells (F, p = 0.39 by Mann-Whitney test).