| Antibodies |
| αCD35, clone 8C12, in BV421 |
BD Biosciences |
Cat#740029; RRID: AB2739801 |
| αCD16/CD32, clone 93 |
ThermoFisher |
Cat#14-0161-85; RRID: AB_467133 |
| αCD45R/B220, clone RA3-6B2, in PE/Cy7 |
BioLegend |
Cat#103222; RRID: AB_313005 |
| αCD4, clone RM4-5, in BV711 |
BioLegend |
Cat#100550; RRID: AB_2562099 |
| αGL7, clone GL7, in PerCP/Cy5.5 |
BioLegend |
Cat#144610; RRID: AB_2562979 |
| αCD38, clone 90, in AF488 |
BioLegend |
Cat#102714; RRID: AB_528796 |
| αC3, clone 11H9 |
Abcam |
Cat#ab11862; RRID: AB_1119819 |
| α-sialic acid, polyclonal |
LSBio |
Cat#LS-C664155 |
| Chemicals, peptides, and recombinant proteins |
| Recombinant mouse MBL2 |
R&D Systems |
Cat#2208-MB-050/CF |
| Recombinant HPV16 L1 |
Abcam |
Cat#ab119880 |
| Recombinant HBsAg AD |
Abcam |
Cat#ab193473 |
| Cholesterol (ovine) |
Avanti Polar Lipids |
Cat#700000 |
| 16:0 PC (DPPC) |
Avanti Polar Lipids |
Cat#850355 |
| N-Decanoyl-N-methylglucamine (MEGA-10) |
Sigma-Aldrich |
Cat#D6277 |
| Quil-A |
Invivogen |
Cat#vac-quil |
| LIVE/DEAD fixable aqua dead cell stain kit |
ThermoFisher |
Cat#L34957
|
| PNGase F |
New England Biolabs |
Cat#P0704S |
| Endoglycosidase H |
New England Biolabs |
Cat#P0702S |
| α2–3,6,8,9 Neuraminidase A |
New England Biolabs |
Cat#P0722S |
|
Erythrina cristagalli gel immobilized lectin |
EY Laboratories |
Cat#A-5901-2 |
| D-lactose monohydrate |
Sigma-Aldrich |
Cat#61345 |
| Critical commercial assays |
| Limulus amebocyte lysate assay |
Lonza |
Cat#QCL-1000 |
| Cholesterol quantification kit |
Sigma-Aldrich |
Cat#MAK043 |
| Alexa Fluor 647 antibody labeling kit |
ThermoFisher |
Cat#A20186
|
| Experimental models: Cell lines |
| Expi293F cells |
ThermoFisher |
Cat#A14527 |
| FreeStyle 293-F cells |
ThermoFisher |
Cat#R79007
|
| Experimental models: Organisms/strains |
| Mouse: wild-type: C57Bl/6J |
The Jackson Laboratory |
JAX: 000664 |
| Mouse: MBL KO: B6.129S4-Mbl1tm1KataMbl2tm1Kata/J |
The Jackson Laboratory |
JAX: 006122 |
| Mouse: C3 KO: B6;129S4-C3tm1Crr/J |
The Jackson Laboratory |
JAX: 003641 |
| Mouse: Cr1/2 KO: B6.129S7(NOD)-Cr2tm1Hmo/J |
The Jackson Laboratory |
JAX: 008225 |
| Mouse: wild-type: B6129SF2/J |
The Jackson Laboratory |
JAX: 101045 |
| Mouse: μIgM KO: B6;129S4-Ighmtm1Che/J |
The Jackson Laboratory |
JAX: 003751 |
| Recombinant DNA |
| pHLSec-eOD |
Jardine et al. (2013)
|
N/A |
| pHLSec-eOD-60mer |
Jardine et al. (2013)
|
N/A |
| pCMV/R-mcs-HA-8mer |
Kanekiyo et al. (2013)
|
N/A |
| pET29b-I53-50A |
Bale et al. (2016)
|
N/A |
| pET29b-I53-50B.4PT1 |
Bale et al. (2016)
|
N/A |
| pCMV/R-I53-50A_4gly |
This paper |
N/A |
| Software and algorithms |
| Prism version 9.2.0 |
GraphPad |
https://www.graphpad.com/scientific-software/prism/
|
| Octet Data analysis software version 8.1 |
ForteBio |
https://www.sartorius.com/en/products/protein-analysis/octet-systems-software
|
| FACSDiva |
BD Biosciences |
https://www.bdbiosciences.com/en-eu/products/software/instrument-software/bd-facsdiva-software#Overview
|
| FlowJo version 10 |
BD Biosciences |
https://www.flowjo.com/solutions/flowjo
|
| ImageJ |
NIH |
https://imagej.nih.gov/ij/
|
