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. 2021 Dec 15;7(1):253–262. doi: 10.1021/acssensors.1c02232

Figure 2.

Figure 2

Validation of anti-GFAP and the detection of GFAP in seven patient plasma samples using ELISA (six patient samples and one healthy control). (A) Comparison between mean ± standard deviation (SD) of optical densities (OD) at 450 nm absorbance in assay buffer solution (pale orange) and control plasma solution (pale green) when spiked with known concentrations between 0.02 and 2.0 ng/mL of GFAP. All concentrations were measured in duplicate. ns = p > 0.05; * = 0.01 < p < 0.05. (B) Concentrations of GFAP detected in the healthy control sample (PS0) and six patient samples (PS1–PS6) measured using ELISA (left axis), and the assay LODs (right axis). All samples were measured in duplicate.