Figure 5.

ArcN AngII increases LSNA and SSNA in part by increasing PVN MC4R sympathoexcitation. Representative experiments (left two columns) and grouped data (right column) showing that prior blockade of PVN MC4R with bilateral nanoinjections of SHU9119 completely prevents ArcN AngII sympathoexcitation (n = 5), whereas PVN SHU9119 has no effects when followed by ArcN aCSF (n = 5). The first open arrow represents the time of PVN bilateral injections of SHU9119 and the second open arrow represents the time of the second ArcN injections (AngII or aCSF). Gray triangles: PVN SHU9119 + ArcN AngII; open squares: PVN SHU9119 + ArcN aCSF. Error bars represent SEM.